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Prevalence, Risk Factors and Molecular Epidemiology of Methicillin Resistant Staphylococcus aureus (MRSA) in Outpatients

Jason Farley

Jason Farley
Principal Investigator

Aim

To characterize the epidemiology and molecular epidemiology of MRSA among an outpatient cohort of persons with HIV and AIDS.

Meet the Investigators<
 

Method

Research Questions:

  1. What is the prevalence of and risk factors for MRSA colonization by anatomical location (i.e. nares, throat, axillae, groin, perineum, rectum and vagina) among a cohort of persons with HIV and HIV/AIDS?

  2. What are the molecular characteristics (e.g. HA-MRSA versus CA-MRSA) and antimicrobial susceptibilities of both the clinical and colonizing isolates?

  3. What is the prevalence, risk factors and molecular characteristics for MRSA of the primary sexual partner of enrolled subjects?

This study will determine the prevalence of MRSA among HIV positive patients by assessing MRSA colonization at multiple body sites. This study will use a technology that combines conventional PCR techniques with electrospray ionization (ESI) mass spectrometry (MS) to perform the molecular analysis. This novel pathogen detection system will provide accurate mass measurements of PCR products and base composition signature analysis to rapidly identify MRSA and differentiate among strain types while simultaneously determining virulence and resistance genes markers such as toxic shock syndrome toxin (TSST-1) and Panton Valentine Leukocidin (PVL). The recognized MRSA clones to date can be distinguished in hours compared to days or weeks for conventional testing.