Douglas A. Granger, a widely known psychoneuroendrocrinology researcher , will join the faculty of Arizona State University in August. Granger has been appointed the ASU Foundation Professor of Psychology and inaugural director of the Institute for Interdisciplinary Salivary Bioscience research. He begins his new position in August and will hold adjunct appointments at the Johns Hopkins University schools of nursing and public health.
Throughout his career, Granger, who is leaving his post as a professor of nursing, public health and medicine at Johns Hopkins University, has pioneered the integration of salivary biomarkers and analytes into basic developmental science. He has studied hormone-behavior relationships across the lifespan (infants, children, adults and the elderly), in extremely high risk and clinical samples (clinic-referred children, maltreated and abused adolescents, victimized children, mentally ill patients, mothers and infants in the context of rural poverty), as well as in low risk (head start) and normative samples.
Previously, Granger was on the faculty of Pennsylvania State University in the Departments of Biobehavioral Health; Human Development and Family Studies. In addition to working in academia, Granger is the founder and chief scientific and strategy advisor for the company, Salimetrics.
The mission of the Johns Hopkins University School of Nursing Center for Interdisciplinary Salivary Bioscience Research is to push the cutting edge of knowledge related to discovery and application of saliva as a research and diagnostic specimen.
To incorporate the measurement of indicators found in oral fluids into research—developmental, social, behavioral, health, clinical, prevention, and rehabilitative sciences—and determine whether our understanding in those areas can be advanced using unique, minimally invasive measurements of biological systems through saliva.
To establish collaborative programs to evaluate the potential value of new biomarkers and analytes present in oral fluids.
To open windows of opportunity for researchers in economics, psychology, sociology, anthropology, gerontology, nursing, social work, preventive medicine, dentistry, medicine, occupational science, sports medicine, psychiatry, and neuroscience.
To create within the Johns Hopkins academic community an environment that supports and enables access to specialized training and laboratory services in salivary bioscience, and which supports researchers in their quest to obtain funding.
To serve as a world stage for investigators to meet, discuss, and refine their ideas about the role of saliva as a research and diagnostic specimen.
The minimally-invasive nature of saliva sample collection, and the broad range of potential measurements, enables oral fluids to be employed in a wide range of fields and disciplines. These features open a window of opportunity for researchers from traditionally non-biologically oriented fields economics, psychology, sociology, anthropology, gerontology, nursing, social work, preventive medicine, dentistry, medicine, occupational science, sports medicine, psychiatry, neuroscience, and more.
In the history of science, major advances are often made at the interface created by interdisciplinary integration. A core mission of the Center for Interdisciplinary Salivary Bioscience Research is to create that opportunity and enable access so that researchers in traditionally non-biologically oriented fields of study can explore this interface.
Evaluating the Potential of Saliva as a Research Specifmen for Use in the National Children's Study
Investigator: Douglas Granger, PhD
Project Description: Saliva, blood, and urine samples will be collected from men and women, mothers and children, in order to evaluate the feasibility of utilizing saliva tests in the National Children's Study (NCS), which will study the effects that a broad range of environmental exposures may have on the growth, development, and health of children in the US. If saliva tests prove to be as feasible as blood and/or urine tests, it is thought that this may increase the participation of mothers and children in the NCS. Both correlation with blood/urine tests as well as general practicality of proper collection by participants will be analyzed.
Assessment of the Impact of Sunflower Oil Emollient Therapy on Neonatal Skin Barrier Function, Nutritional Status, Bacterial Colonization of the Skin, and Markers of Immune Response in Sarlahi, Nepal
Investigator: Luke Mullany, PhD, MHS
Project Description: This project will take place over about 12 months and sample 500 preterm and 500 fullterm babies in Sarlahi District in rural Nepal to determine the possible mechanistic reasons for improved health outcomes in neonates receiving full body massage using sunflower seed oil relative to mustard seed oil. These activities broadly relate to four domains: improved skin integrity and function, microbial challenge at the skin, biomarkers of immune responses, and nutritional status.
This project will specifically examine whether: trans-epidermal water loss is decreased; skin pH more rapidly stabilizes during the first week of life; there is a difference in stratum corneum protein content and amount; skin condition scores for dryness, rash, and erythema are lower (improved) among babies in the sunflower oil group; overall and organism-specific presence and density of colonization at the axilla, inguinal, and periumbilical region differs between the groups; there are changes in immune response at both the systemic and epidermal innate immunity levels; there are differences in salivary and serum cytokine concentrations over the first week of life using repeat salivary swabs and heelprick blood samples; there are differences in epidermal biomarkers (keratin 1,10,11, involucrin, albumin) and cytokine levels (IL-1α, IL-1β, IL-6, IL-8, TNF-α) using a non-invasive skin tape-strip sampling method; weight is higher 7, 28, and 180 days after birth; length is greater at 7, 28 and 180 days after birth; and serum levels of essential fatty acids are improved among babies in the sunflower oil group.
Fetus to Five Study, Funded by NIDA
Project Description: The Fetus to Five Study is focused on understanding the development of self-regulation from the prenatal period to age 5. Part of this study is focused on understanding how social experiences shape the development of physiological processes that support cognitive, behavioral, emotional, and physiological self-regulation. Salivary biomarkers are being used to provide a "snapshot" of functioning across a variety of body systems both in the lab and in participating families' daily lives.
Pediatric Asthma Alert Intervention (PAAL)
Investigator: Arlene Butz, ScD, MSN, CPNP
Project Description: This is a randomized controlled trial of a clinician and parent feedback intervention for children with persistent asthma and with frequent emergency department (ED) visits for asthma. The specific aim is to determine if providing the child's primary care provider (PCP) and parent with asthma health information, including cotinine concentrations as a biomarker of second hand smoke exposure, is effective in reducing subsequent ED asthma visits. Overall 300 children have been recruited and are currently being followed for 12 months. Salivary cotinine concentrations are collected at baseline and 12 months post randomization to determine if providing the parent with a child's cotinine level will motivate the family to institute a total home smoking ban and reduce the child's exposure to second hand smoke and reduce ED utilization.
Preventing Respiratory Infections During Early Childhood (PRIDE)
Investigator: Cynthia Rand, PhD
Project Description: The purpose of the PRIDE study is to conduct a randomized clinical trial of a home-delivered, motivational interviewing-based secondhand smoke (SHS) reduction intervention combined with a Head Start (HS) -level education program in reducing children's SHS exposure, compared to a HS-level education program alone. We will enroll 350 Head Start students aged 2-5 years with a caregiver-reported smoker in the home from all 17 Baltimore City HS programs. The primary study outcome measure will be household SHS levels, as measured by home air nicotine levels at six month follow-up. Secondary outcomes include caregiver-reported home and car smoking bans, child's salivary cotinine levels, children's respiratory symptoms, caregiver smoking cessation, school absences, and health care utilization measures.
Urasche, A., Blair, C., Granger, D. A., Stifter, C., Voegtline, K., and the Family Life Project Investigators (accepted, minor revisions). Behavioral reactivity to emotion challenge is associated with cortisol reactivity and regulation at 7, 15, and 24 months of age. Developmental Psychobiology.
Johnson, S. B., Riley, A. W., Granger, D. A., & Riis, J. L. (in press). The science of early life toxic stress for pediatric practice and advocacy. Pediatrics. PMID 23339224
Savla, J. T., Granger, D. A., Roberto, K. A., Davey, A., Blieszner, R., & Gwazdauskas, F. (accepted, minor revisions). Cortisol and Alpha Amylase Reactivity to Care-related Stressors in Spouses of Persons with Mild Cognitive Impairment. Psychology and Aging.
Francis, L. A., Granger, D. A. & Susman, E. J. (in press). Adrenocortical regulation, eating in the absence of hunger and BMI in young children. Appettite. PMID 23219991
Taylor, Z. E., Spinrad, T. L., VanSchyndel, S., Eisenberg, N., Huynh, J., Sulik, M. J., & Granger, D. A., (in press). Sociodemographic risk, parenting, and effortful control: Relations to salivary alpha-amylase and cortisol in early childhood. Developmental Psychobiology. PMID 22949301
Out, D., Granger, D. A., Sephton, S. E., & Segerstrom, S. C. (in press). Disentangling sources of individual differences in diurnal salivary alpha-amylase: Reliability, stability, and sensitivity to context. Psychoneuroendocrinology. PMID 22819683
Dreschel, N., A., & Granger, D. A. (in press). Advancing the social neuroscience of human-animal interaction: The role of salivary bioscience. In “The Social Neuroscience of Human –Animal Interaction" (Eds., L. Freund,S. McCune,P. McCardle, L. Esposito & J. Griffin, Eds).
Feinberg, M., Jones, D., Granger, D. A. & Bontempo, D. (in press). Anxiety and chronic couple relationship stress moderate adrenocortical response to couple interaction in expectant parents. British Journal of Psychology.
Wilde, C, Out, D., Johnson, S., & Granger, D. A. (in press). Sample collection, including participant preparation and sample handling. In D. Wild (ed.), The Handbook of Immunoassay, Elsevier, London.
Scott, L. N., Levy, K. N., & Granger, D. A. (in press). Biobehavioral reactivity to social evaluative stress in women with borderline personality disorder. Personality Disorders: Theory, research and treatment. PMID 23244772
Granger, D.A., Johnson, S. B., Szanton, S. L., Out, D., Lau Schumann, L., (in press). Incorporating salivary biomarkers into nursing research: an overview and review of best practices. Biological Research in Nursing, 14, 347-356. PMID 22593229
Middlemiss, W., Granger, D. A., & Goldberg, W. A. (2013). Response to “Let’s help parents help themselves: A letter to the editor supporting the safety of behavioural sleep techniques. Early Human Development, 89, 41-42. PMID 22889752
McGraw, L. K., Loan, L. A., Hammermeister, J. J., Ohlson, C. J., Out, D., & Granger, D. A. (2013). Biobehavioral reactivity and regulation in Army Nurses in response to a combat casualty simulation stress task, 38, 135-144. Psychoneuroendocrinology. PMID 22710003
Berry, D., Blair, C. Willoughby, M., Granger, D. A., and the FLP Investigators (2012). Salivary alpha-amylase and cortisol in infancy and toddlerhood: Direct and indirect relations with executive functioning and academic ability in childhood. Psychoneuroendocrinology,37, 1700-1711 PMID 22472478
Bright, M., Frick, J., & Granger, D. A. (2012). Do young children show a cortisol awakening response?. Developmental Psychobiology, 54, 736-743 PMID 22006547.
Granger, D. A., & Johnson, S. B., (2012). Salivary Biomarkers. In M. D. Gellman & J. R. Turner (eds.), Encyclopedia of Behavioral Medicine,pp 1005-9, Springer, New York
Byrd-Craven, J., Auer, B. J., Granger, D. A., & Massey, A. R. (2012). The father-daughter dance: The influence of the father-daughter relationship quality on daughters’ stress response to peer dynamics. Journal of Family Psychology, 26, 87-94 PMID 22182338
Middlemiss, W., Granger, D. A., Goldberg, W. A., & Nathans, L. (2012). Asynchrony of mother-infant hypothalamic-pituitary-adrenal axis activity following extinction of infant crying responses Induced during the transition to sleep. Early Human Development, 88, 227-232 PMID 21945361
Kreher, D. A., Powers, S. I., & Granger, D. A. (2012). Relationship between cortisol, salivary alpha-amylase, and cognitive bias in young women. Behavioral Neuroscience, 126, 157-166. PMID 22289045
Keller, P. S., El-Sheikh, M., Granger, D. A., & Buckhalt, J. A. (2012). Interactions between salivary cortisol and alpha-amylase as predictors of children’s cognitive functioning and academic performance. Physiology and Behavior. 105, 987-995. PMID 22100627
Vermeer, H. J., Van Ijzendoorn, M. H., Groeneveld, M. G., and Granger, D. A. (2012). Downregulation of the immune system in low-quality day care: The case of secretory immunoglobulin A (SIgA) in toddlers. Physiology and Behavior, 105, 161-167
Nemoda, Z.., Horvat-Gordon, M., Fortunato, C. K., Beltzer, E., Scholl, J. L., Granger, D. A. (2011). Assessing genetic polymorphisms using DNA extracted from cells present in saliva Samples. BMC Medical Research Methodology, 11, 170.
Out, D., Bakermans-Kranenburg, M. J., Granger, D. A., Cobbaert, C. M., & Van IJzendoorn, M. H. (2011). State and trait variance in salivary alpha-amylase during baseline and stress: A behavioral Genetic Study. Biological Psychology, 88, 147-154
Dorius, C., Booth, A.,Hibel, J., Granger, D. A., and Johnson, D. (2011). Parents’ Testosterone and children’s perception of parent-child relationship quality. Hormones and Behavior, 60, 512-519
Adam, E. K., Hoyt, L. T., & Granger, D. A. (2011). Diurnal alpha amylase patterns in adolescents and associations with momentary mood states. Biological Psychology, 88, 170-173.
Allwood, M. A., Handwerger, K., Kivlighan, K. T., Granger, D. A., & Stroud, L. R. (2011). Direct and moderating links of saliva alpha amylase and cortisol stress-reactivity to youth behavioral and emotional adjustment. Biological Psychology, 88, 57-64
Feinberg, M., Jones, D. E., Granger, D. A. & Bontempo, D. (2011). Relation of Intimate partner violence to salivary cortisol among couples expecting a first child. Aggressive Behavior, 37, 492-502
Afifi, T. D., Granger, D. A., Denes, A., Joseph, A., Aldeis, D. (2011) Parent’s communication skills and adolescents’ salivary alpha-amylase and cortisol response patterns. Communication Monographs, 78, 273-295.
Hibel, L. C., Granger, D. A., Blair, C., Cox, M. J., & The Family Life Project Key Investigators (2011). Maternal sensitivity buffers the adrenocortical implications of intimate partner violence exposure during early childhood. Development and Psychopathology,23, 689-701..
Blair, C., Raver, C.C., Granger, D. A., Mills-Koonce, R., Hibel L., and the Family Life Project Key investigators (2011). Allostasis and allostatic load in the context of poverty in early childhood. Development and Psychopathology, 3, 845-857
Clark, F., Jackson, J., Carlson, M., Chou, C-P., Cherry, B., Jordan-Marsh, M., Knight, B. G., Mandel, D., Blanchard, J., Granger, D. A., Lai, M.Y., White, B., Forman, T., Hay, J., Lam, C. , Marterella, A., & Azen, S. P. (in press). Effectiveness of a lifestyle intervention in promoting the well being of independently living older people: Results of the well elderly II randomized clinical trial. Journal of Epidemiology and Community Health
Blair, C., Granger, D. A., Willoughby, M., Mills-Koonce, R., Cox, M., Greenberg, M. T., Kivlighan, K., Fortunato, C. & The FLP investigators (2011). Salivary cortisol mediates effects of poverty and parenting on executive functions in early childhood. Child Development, 82, 1970-1984
Glenn, A, L., Raine, A., Schug, R. A., Gao, Y., & Granger, D. A. (2011). Increased testosterone to cortisol ratio in psychopathy. Journal of Abnormal Psychology, 120, 389-399
Mills-Koonce, R., Garrett-Peters, P., Barnett, M., Granger, D. A., Blair, C., Cox, M., & the Family Life Project Key Investigators (2011). Father contributions to cortisol responses in infancy and toddlerhood. Developmental Psychology, 47, 388-395
Rudolph, K. D., Troop-Gordon, W., & Granger D. A. (2011). Individual differences in biological stress responses moderate the contribution of early peer victimization to subsequent depressive symptoms. Psychopharmacology, 214, 209-219.
Eiden, R. D., Granger, D. A., Schuetze, P., & Veira, Y. (2011). Child Behavior Problems Among Cocaine-Exposed Toddlers: Indirect and Interactive Effects.Development and Psychopathology, 23, 187-198
Byrd-Craven, J., Granger, D. A., & Auer, B. J. (2010). Stress-reactivity to co-rumination in young women’s friendships: Cortisol, alpha-amylase, and negative affect focus. Journal of Personal and Social Relationships, 28, 469-487
Keller, P. S., El-Shiekh, M., Vaughn, B., & Granger, D. A. (2010). Relations between mucosal immunity and children’s mental health: The role of Child Sex. Physiology & Behavior,101,705-712
Yim, I. S. Granger, D. A., & Quas, J. (2010). Children’s and adults alpha-amylase responses to a laboratory stressor and to verbal recall of the stressor. Developmental Psychobiology, 52, 598-602
Rudolph, K. D., Troop-Gordon, W., & Granger D. A. (2010). Peer victimization and aggression. Moderation by individual differences in salivary cortisol and alpha-amylase. Journal of Abnormal Child Psychology,38, 843-856
Beltzer, E., Fortunato, C. K., Guaderramma, M., Peckins, M. K., Garramone, B. M., & Granger, D. A. (2010). Salivary flow and salivary alpha-amylase: Collection technique, duration, and oral fluid type. Physiology and Behavior, 101, 289-296
Klein, L. C., Whetzel, C. A., Bennett, J. M., Granger, D.A. & Ritter, F. E. (2010). Caffeine and stress alter salivary alpha-amylase activity in young men. Human Psychopharmacology Clinical and Experimental, 29, 359-367.
Vigil, J. M., Geary, D. C., Granger, D. A., & Flinn, M. V. (2010). Sex differences in salivary cortisol, alpha-amylase, and psychological functioning following Hurricane Katrina. Child Development, 81, 1227-1239
Carney, J-L., Hazler, R. J., Oh, I., Hibel, L. C., & Granger, D. A. (2010). The relationships between bullying exposures in middle childhood, anxiety, and adrenocortical activity. Journal of School violence, 9, 194-211
Susman, E. J., Dockray, S., Granger, D. A., Blades, K.T., Randazzo, W. T., Heaton, J. A., & Dorn, L. D. (2010). Cortisol and alpha amylase reactivity and timing of puberty: Vulnerabilities for antisocial behavior in young adolescents. Psychoneuroendocrinology, 35, 557-569.
Gordis, E. B., Margolin, G., Spies, L., Susman, E . J. & Granger, D. A. (2010). Interparental aggression and parent-adolescent salivary alpha amylase symmetry. Physiology and Behavior, 100, 225-233
Fisher, A. J., Granger, D. A., & Newman, M. G. (2010). Sympathetic arousal moderates self-reported trait autonomic arousal at baseline and physiological flexibility in response to a stressor in generalized anxiety disorder. Biological Psychology, 83, 191-200
Reported on January 17, 2013 in Johns Hopkins Medicine News
It’s no fun getting stuck with a needle at the doctor’s office, but drawing blood is often the only way to detect illness, test the effectiveness of medicines and evaluate organ function. That may be changing, however, thanks to the research of Johns Hopkins University professor Doug Granger, Ph.D., who has spent the past 20 years looking at how to use a saliva swab as an alternative for blood collection.
Reported August 23, 2012 on University of Nebraska-Lincoln Building Research Collaborations website
CISBR director Douglas Granger delivered the keynote speech for the East Stadium Research Facility Expansion Project at the University of Nebraska-Lincoln. Part of the facility will be dedicated to the study of biomarkers.
But it's the only way doctors can get blood to test for diabetes, anemia and numerous other health problems.
Scientists at the Johns Hopkins University School of Nursing say there is a much less invasive and painless means of detecting illnesses in patients — spit.
Reproduced from Johns Hopkins Nursing, Fall/Winter 2010
Researchers are constantly finding new ways to figure out what makes us human beings tick, and one of the newer methods makes you want to spit—literally.
Saliva is full of analytes and biomarkers that create a biological journal of exposure to chemicals and disease, and of genetic variability. However, the collection of oral fluid has always proved cumbersome, with researchers depending on swabs or collection cups.
A new tool developed by the Johns Hopkins University School of Nursing Center for Interdisciplinary Salivary Bioscience Research (CISBR) in collaboration with SalivaBio, LLC, improves the ease of oral fluid collection, while maintaining the integrity of the biospecimen.
America’s children and teens are gaining…weight. As many as 16.9 percent of children ages 2-19 are obese; another 14.8 percent are overweight, placing them at risk for later, chronic problems ranging from diabetes and sleep apnea to depression and heart disease.
Anywhere it wants. OK, they don’t really expectorate. So Johns Hopkins University School of Nursing (JHUSON) saliva expert Douglas Granger has done a bit of improvisation.
Studies at the Johns Hopkins University School of Nursing are on the cutting edge of community-based research addressing healthcare and health disparities among African Americans and other medically underserved groups.
Can modern technology be the solution to the ever-increasing problem of overworked and overstressed college students?
Douglas Granger, professor and director of the Johns Hopkins University School of Nursing Center for Interdisciplinary Salivary Bioscience Research, is taking his now-famous “Spit Camp” to the University of California Irvine, April 2-3.
Spit is central to the conversation for salivary researchers at the Johns Hopkins University School of Nursing, who are discovering new ways to keep people healthy and well.
Hopkins Nursing researchers focus on stress, parenting programs, diabetes, and more in the January issue of research news.
Researchers are constantly finding new ways to figure out what makes us human beings tick, and one of the newer methods makes you want to spit—literally.
The rapid accumulation of knowledge generated by the application of salivary analytes creates special opportunities. Yet, the cutting edge information is often presented across a variety of scientific meetings and journals. Thus, it can be challenging to stay up to speed with the most current information.
On a regular basis, the Center will serve as a forum for the presentation and discussion of the state of knowledge about salivary analytes and their applications. The Center will host small conferences and forums with the goal of defining boundaries of, and gaps in our knowledge, and charting future directions to enable the establishment of scientific critical mass.
Much of the work on salivary analytes to date has focused on just a handful of measures related to infectious disease exposure and endocrine processes. A primary objective of the Center is to reveal the potential of this library of analytes by developing methods to measure them, and then documenting the value of these "new" salivary analytes for the behavioral, developmental, and health sciences.
In addition to the analytes below, CISBR is pleased to be able to offer genotyping services in conjunction with the Johns Hopkins School of Public Health Genomic Analysis and Sequencing Core.
|Current Analytes||Analytes Under Development|
Salivary oxytocin analysis services are temporarily suspended to assess accurracy of measurement. The center is partnering with Salimetrics1 to develop a new assay, however it may take several months before reaching completion. We apologize for the inconvenience.
For inquiries and/or price quote, email firstname.lastname@example.org.
To submit samples for assay, send us a Project Overview and we will send you a Testing Order Form (TOF) and Sample Receiving Manifest (SRM). The TOF and SRM must be submitted at least 24 hours prior to the planned shipping date.
1In the interest of full disclosure, Dr. Granger is founder and Chief Scientific and Strategy Advisor of Salimetrics LLC (State College, PA), and this relationship is managed by the policies of the Johns Hopkins University School of Medicine Conflict of Interest Committee.
You are invited to join us for Spit Camp, a two-day workshop program designed for faculty, post-doctoral scholars, and fellows. Lectures and discussions are led by Dr. Granger, and laboratory activities are led by Jessica Parker. Attendance is limited to four to five participants per camp session to allow for in-depth, individualized discussion and instruction.
The lecture component covers theoretical perspectives; oral fluid as biological specimens; practical aspects of sample handing, collection, and study design; basics of immunoassay used for assaying saliva; and hints for writing papers, presentations, and proposals. The laboratory component includes hands-on supervised training on sample processing, salivary immunoassay, and kinetic reaction assays. Samples will be collected, assayed, and data generated for discussion and analysis.
"I learned so much about saliva collection, analyses, and interpretation. I also learned the processes behind obtaining saliva data, which helps me in research design, implementation, as well as later write up. Finally, perhaps most importantly, I learned what areas of saliva data related research is lacking and what future directions look like, which, as a graduate students, gives me plenty of good ideas about how to make my research unique."
"I gained a better understanding of how to collect saliva samples and of potential challenges in doing so, more knowledge of the process of conducting salivary assays, and an introduction to relevant theory and empirical findings. I also gained a better understanding of how to conceptualize studies and how to think about what we can learn from salivary analytes."
"I would certainly recommend this workshop, especially to researchers planning a study or to research team members who will be collecting saliva samples."
There are no upcoming spit camp workshops scheduled at Johns Hopkins.
University of California, Irvine
Contact the Center at email@example.com for more information about availability and scheduling.
CISBR will hold a salivary bioscience symposium at Johns Hopkins Medical Campus in Baltimore. More details to follow!
Contact the Center: firstname.lastname@example.org | 443-287-6545